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Electroporation buffer, isoosmolar

Electroporation buffer, isoosmolar
Your Price: $63.00
Item No.: 4094-2109
Electroporation buffer, isoosmolar, 100 ml, sterile

The electroporation buffer system used with the Multiporator contributes towards increased efficiency. The use of these buffers (hypo and isoosmolar) with low conductivity prevents cell damage by reducing current flow. In hypoosmolar conditions, cells swell and the cell membrane becomes detached from the cytoskeleton, thereby enabling easier electroporation. Ideally, electroporation is carried out in hypoosmolar electroporation buffer; for very sensitive cells the buffer system can be diluted with isoosmolar electroporation buffer.

  • The hypoosmolar buffer system swells the cell for easier membrane penetration
  • The ion composition is adapted to the inner cell environment, stabilizing the NA/K gradient across the cell membrane

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